Scientist - Biology Job Responsibilities Design, develop, and optimize in vitro and biochemical (cell-based and purified protein) assays to characterize drug–target interactions for target validation, compound screening, and mechanism-of-action studies. Apply equilibrium/kinetic binding models to derive parameters (Kd, Ki, kon, koff, residence time, stoichiometry) using radiometric and fluorescence platforms. Plan experiments to distinguish specific vs. non-specific binding; control receptor expression and probe depletion/rebinding. Analyze cellular target engagement data (structure-function, conformational dynamics, MoA). Use statistical/computational methods to ensure assay robustness and reproducibility (e.g., Z’ factor, CV, signal window). Translate scientific questions into robust assays; lead projects supporting the biology screening/evaluation plan and collaborate with chemists, DMPK, imaging, and in vivo teams. Maintain documentation; safely work with radioactive isotopes per lab/regulatory guidelines. Qualifications & Skills (Required/Preferred) Ph.D. in Cancer Biology/Cellular & Molecular Biology (or related). Strong hands‑on assay development/optimization; proficiency with cell culture and molecular biology; experience with ELISA, pathway assays, flow cytometry, western blot, TR-FRET/HTRF, competitive binding, and/or high‑content imaging. Ability to work in interdisciplinary teams; excellent communication/detail; radiation safety; AI tools to improve productivity/quality. Preferred: industry/academic drug discovery experience; handling radioactive materials; high‑throughput/automated binding assays; additional biophysics (SPR/BLI/ITC/MST); live‑cell engagement platforms (NanoBRET, CETSA); receptor trafficking/membrane biology. Benefits/Compensation (if stated) San Diego, CA: $118,259–$143,302; health coverage; wellbeing/EAP; 401(k) and insurance/disability; paid time off. #J-18808-Ljbffr